Rami Arieli: "The Laser Adventure" Chapter 2.8 page 2
Conclusion:
If at specific moment (t=0) the number of atoms in the excited state E2 is N2(0), then when we leave the system without external influence, the number of atoms in the excited state will decay exponentially according to the equation in previous page.

Pay attention that in spontaneous emission, the population number (N1) of the lower energy level (E1) is unimportant.